Перегляд за автором "Okunev, O.V."

Результати 1-5 з 5

Bioaffinity sorbent based on immobilized protein A Staphylococcus aureus: development and application

Gorbatiuk, O.B.; Tsapenko, M.V.; Pavlova, M.V.; Okunev, O.V.; Kordium, V.A. (Вiopolymers and Cell, 2012)

Цель. Создание биоаффинного сорбента на основе ориентированно иммобилизованного белка А Staphylococcus aureus (SPA) через два целлюлозосвязывающих домена (СBD) Clostridium thermocellum и его применение для очистки антител. ...
Construction, expression, functional characterization and practical application of fusion protein SPA-BAPmut

Gorbatiuk, O.B.; Okunev, O.V.; Nikolaev, Yu.S.; Svyatenko, O.V.; Kordium, V.A. (Вiopolymers and Cell, 2013)

Aim. The creation of genetically engineered fusion protein SPA-BAPmut and its application as a secondary immunoreagent in immunoassays. Methods. Gene cloning, PCR, electrophoresis, DNA sequencing, bacteria cells culturing, ...
Development of the chromatographic medium for the affinity isolation of the recombinant hIFN-β1b based on immobilized single-chain antibodies

Pokholenko, Ia.O.; Gorbatiuk, O.B.; Okunev, O.V.; Irodov, D.M.; Degtiarova, M.I.; Palivoda, O.G.; Kordium, V.A. (Вiopolymers and Cell, 2015)

Aim. The development of a laboratory method for the production of immunoaffinity chromatography medium for the purification of the recombinant human IFN-β1b. Methods. A gene of the chimeric protein ScFvbINF-CBD was constructed ...
Molecular mechanisms of versatile biological activity of interleukin-7

Slyvka, A.V.; Okunev, O.V. (Вiopolymers and Cell, 2014)

As a result of immunological studies of the last 25 years it turned out that interleukin-7 (IL-7) is one of the most important regulatory cytokines of the immune system. It is special in many concerns. Having a strong ...
Recombinant Staphylococcal protein A with cysteine residue for preparation of affinity chromatography stationary phase and immunosensor applications

Gorbatiuk, O.B.; Bakhmachuk, A.O.; Dubey, L.V.; Usenko, M.O.; Irodov, D.M.; Okunev, O.V.; Kostenko, O.M.; Rachkov, A.E.; Kordium, V.A. (Вiopolymers and Cell, 2015)

Aim. Engineering of recombinant Staphylococcal protein A with cysteine residue (SPA-Cys) for preparation of affinity chromatography stationary phase and formation of bioselective element of immunosensor. Methods. DNA ...

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Перегляд за автором "Okunev, O.V."

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